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医学论文范文:阿加曲班对大鼠肢体缺血再灌注损伤血液流变学指标影响的实验研究
【摘要】 目的 探讨阿加曲班对大鼠肢体缺血再灌注损伤对血液流变学指标的影响，为缺血再灌注损伤的防治提供新思路。方法 采用大鼠肢体缺血再灌注模型，将14只Wistar大鼠随机分为2组，对照组和阿加曲班组，每组7只。2组大鼠均于再灌注2 h后，酶联免疫吸附(ELISA)法测定血浆P选择素、细胞间粘附分子1(ICAM1)浓度;取血检测血液流变学指标,包括血浆粘度(ηp)、全血低切还原粘度(Lηre)、全血高切还原粘度(Hηre)、红细胞压积(HCT)、红细胞聚集指数(EAI)、血沉方程K值(ESRK)、红细胞刚性指数(TK)、红细胞变形指数(EDI)。结果 大鼠肢体缺血再灌注2 h后，阿加曲班组血浆P选择素浓度、血浆ICAM1浓度显著低于对照组(P<0.01)。血液流变学指标与对照组比较，阿加曲班组ηp、Hηre、Lηre、EAI和TK明显降低(P<0.05)，EDI升高(P<0.05)，而对Hct、ESRK无显著影响(P<0.05)。结论 阿加曲班能够降低血浆P选择素和ICAM1浓度，进而改善大鼠肢体缺血再灌注损伤所造成的血液流变学异常。
【关键词】 缺血再灌注损伤;选择素;ICAM1;血液流变学; 阿加曲班
The effects of argatroban on hemorheological parameters in rats with limb ischemiareperfusion injury DU Liqing^,MI Shijun,WANG Jinghua.^Department of Danger and Severe Medicine,The Fengrun Distriact People’s Hospital,Hebei,Tangshan 064000,China
【Abstract】 Objective To investigate the effects of agatroban on hemorheological parameters,plasma Pselectin and plasma ICAM1 of rats with limb ischemia/reperfusion injury in order to provide a new method for the prevention and therapy of ischemia/reperfusion injury. Methods 14 Wistar rats were randomly divided into control group and agatroban group, with 7 rats in each group.The rat model of limb ischemia/reperfusion injury was made according to the ZHANG Lianyuan’s ways. The plasma Pselectin and plasma ICAM1 levels were detected by enzyme linked immunosorbent assay (ELISA) at 2h after reperfusion. The blood samples were taken and hemorheological indexes were detected, including plasma viscosity, low shear reduction viscosity, high shear reduction viscosity, hematocrit erythrocyte aggregation index, erythrocyte sedimentation rate K and erythrocyte rigidity index.Results 2h after limb ischemia/reperfusionin rats,the plasma Pselectin and plasma ICAM1 levels in argatroban group were significantly lower than those of control group(P<0.05).As compared with those in control group,the ηp、Hηre、Lηre、EAI and Tk in argatroban group were significantly decreased(P<0.05),however,EDI was obviously increased(P<0.05),but no obvious effect on Hct,ESRK was observed.Conclusion Agatroban can reduce plasma Pselectin and plasma ICAM1 levels,as a result,to improve hemorheological abnormality caused by limb ischemiareperfusion injury in rats.
【Key words】 ischemiareperfusion injury; hemorheology; argatroban; Pselectin; ICAM1
近年随着对缺血再灌注损伤基础研究的深入，对其再灌注损伤的保护研究也越来越多。试图应用不同的方法，通过不同的途径发挥作用。缺血预处理、抗氧化疗法、抗白细胞疗法等，都有很好的保护作用[1，2]。阿加曲班是化学合成的低分子药物,直接与凝血酶的催化活性位点结合,灭活凝血酶。近年研究表明阿加曲班还可抑制凝血酶的其他作用，如可以抑制肿瘤转移、炎性过程及血管成形术后的再狭窄等[3]，对微循环障碍和血液流变性也有一定影响[4]。但能否对肢体缺血再灌注损伤导致的血液流变学异常有良好的改善作用，我们对大鼠肢体缺血再灌注损伤进行了实验研究，观察对比阿加曲班干预和单纯缺血再灌注损伤导致的血液流变学异常有无显著性影响，为缺血再灌注损伤的临床防治提供实验基础医.学全.在.线网站www.lindalemus.com。
