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医学论文范文:Toll样受体2对角质形成细胞分泌IL8的影响
【摘要】 目的:观察白念珠菌刺激人角质形成细胞后IL8的变化以及Toll样受体2(Tolllike receptor 2，TLR2)对IL8分泌的影响，探讨角质形成细胞在皮肤免疫系统中的作用。方法：白念珠菌分别与抗TLR2抗体预处理前后的角质形成细胞孵育，ELISA方法检测不同时间培养上清液IL8的水平。结果：白念珠菌可引起IL8明显升高(P<0.01)，3h开始，至24h达到高峰;中和TLR2受体后，虽然引起IL8升高,但无统计学意义(P>0.05)。结论：白念珠菌可以刺激角质形成细胞分泌IL8，TLR2在角质形成细胞IL8表达中的作用尚难确定。
【关键词】 白念珠菌 Toll样受体2 IL 8 角质形成细胞 天然免疫
Effects of Tolllike Receptor2 on Interleukin8 Secretion of Keratinocytes HUANG Guangrong1, FAN Li1, DUAN Yingchun1, DUAN Dejian2^ (1Department of Obstetrics and Gynecology,Renmin Hospital;2Department of Dermatovenereology,Taihe Hospital,Yunyang Medical College,Shiyan,Hubei 442000,China)
Abstract: Objective To observe the change of Interleukin8(IL8) in keratinocytes induced with Candida Albicans and the effect of Tolllike receptor2(TLR2) on IL8 secretion,to explore the role of keratinocytes in skin immune system. Methods Candida Albicans were cultured on keratinocytes treated before and after antiTLR2 monoantibody(mAb),respectively.The levels of IL8 in culture supernatant were determined with ELISA at different time points. Results The IL8 levels were significantly increased after induced with Candida Albicans(P<0.01),the significant increase was observed at 3 h and reached the peak at 24 h;the increase of IL8 levels had no significant difference after TLR2 was neutralized with antiTLR2 mAb(P>0.05). Conclusion The secretion of IL8 in keratinocytes could be induced with Candida albicans,the function of TLR2 is uncertain in expression of IL8 in keratinocytes医.学全.在.线网站www.lindalemus.com.
Key words:Candida albicans;Tolllike receptor2;Interleukin8;Keratinocytes;Innate immunity
念珠菌在人体表面的分布广泛而常见，而白念珠菌是引起念珠菌病的主要致病菌种。在去除表皮的实验说明表皮角质形成细胞在抵抗念珠菌感染中起重要作用。皮肤粘膜念珠菌病常表现为病原菌寄生部位的角层下脓疱或角层内大量中性粒细胞聚集，但是否与细胞表达的趋化因子IL8有关，目前还不太清楚[1]。Toll样受体2(Tolllike receptor 2，TLR2)是表达于角质形成细胞并参与真菌产物识别的天然受体蛋白，其与IL8的关系亦研究不多，故本研究旨在探讨TLR2对角质形成细胞分泌IL8的影响。
