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【摘要】 目的 观察川芎嗪对谷氨酸损伤海马神经元的保护作用。方法 原代培养大鼠海马神经元，培养基中加入1mmol/L的盐酸川芎嗪溶液共同孵育12h后，加入1mmol/L谷氨酸损伤海马神经元20min。采用MTT法检测细胞活性;测定培养液中乳酸脱氢酶(LDH )活力的变化;细胞免疫组化检测各组细胞表达AchE。 结果 川芎嗪可提高谷氨酸损伤后海马神经元的存活率，抑制因谷氨酸损伤引起的细胞内LDH的释放，可促进谷氨酸损伤细胞中AChE的表达。 结论 川芎嗪对谷氨酸诱导海马神经元损伤有保护作用。
【关键词】 川芎嗪;谷氨酸;海马神经元
Protective effects of ligustrazine on glutamateinduced
neurotoxicity in cultured hippocampal neuronsBAO Zhijun, XIA Chunlin
(Department of Anatomy and Cytoneurobiology, Medical School of
Soochow University, Suzhou 215123, China)ABSTRACT: Objective To observe the protective effects of ligustrazine on glutamateinduced injury in cultured hippocampal neurons. Methods Primarily cultured hippocampal neurons from fetal rats were incubated with ligustrazine (1mmol/L) for 12 hours, then glutamate (1mmol/L) was added for 20 minutes to induce injury. Cell viability was detected by MTT assay, and the change in LDH activity was determined by biochemical method. Finally, the expression of AchE in the cultured neurons was assayed by immuocytochemistry. Results Ligustrazine improved the survival rate of hippocampal neurons injured by glutamate, inhibited the release of LDH from the kytoplasm injured by glutamate. The expression of AchE in the injured neurons was promoted by pretreatment of ligustrazine. Conclusion Ligustrazine can significantly exert protective effects on the hippocampal neuron injury induced by glutamate.
KEY WORDS: ligustrazine; glutamate; hippocampal neuron医.学全.在.线网站www.lindalemus.com
海马是边缘系统的重要组成部分，参与学习、记忆、情绪反应及植物神经功能等。原代培养的海马神经元对许多致病因素和化学损害较为敏感，是研究神经元损伤及抗损伤的理想模型。在脑缺血模型中，海马神经元的损伤较为严重，其中兴奋性氨基酸含量增高是海马神经元损伤的原因之一。谷氨酸(glutamate, Glu)是哺乳动物脑内主要的兴奋性氨基酸，参与中枢神经系统的信息传递。但Glu过度释放可产生神经兴奋性毒性，引起神经细胞损伤或死亡。川芎嗪(ligustrazine, Lig)是从中药川芎总生物碱中分离得到的一种有效活性生物碱。Lig能通过血脑屏障进入中枢神经系统，在脑中快速达到峰值，并持久地存留在大脑内。临床上常用Lig治疗脑缺血，其机制是通过扩张微血管改善微循环，抑制血栓形成，阻断自由基连锁反应，降低细胞内钙离子超载，拮抗氨基酸毒性等途径来实现，具有多靶点、多层次、多环节的脑保护作用[1]。本研究采用体外海马神经元原代培养，观察Lig对Glu损伤海马神经元的保护作用，探讨Lig对脑损伤神经元的保护机制。
