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医学论文范文:人肾透明细胞癌组织中增殖细胞核抗原表达与肾癌的关系
【摘要】 目的 通过检测人肾透明细胞癌组织中增殖细胞核抗原(PCNA)表达的变化，研究其表达与肾癌的关系。方法 标本离体后分成肾透明细胞癌组、癌旁组织组、正常肾组织组，均用10%的甲醛固定48 h，常规石蜡包埋、切片5 μm，同时应用免疫组织化学方法进行观察研究。结果 PCNA免疫组化染色：正常肾组织内的细胞核有弱阳性表达，癌旁阳性表达增加，高于正常肾组织，肾癌组织阳性表达明显增强， 明显高于癌旁组织，两两比较差异均有统计学意义(P<0.01)。肾癌4个期之间比较差异无统计学意义(P>0.05)。结论 PCNA在正常组、癌旁组、癌组织组的表达呈明显加强趋势，癌组织细胞增殖旺盛，是肿瘤形成的重要机制。
【关键词】 人肾透明细胞癌;增殖细胞核抗原
The expression of proliferating cell nuclear antigen (PCNA ) in the human clear cell renal cell carcinoma ZHANG Jianyong^, LIANG Liang, DONG Xiaoju,et al.^Department of Urology,The Second Hospital of Shijiazhuang,Hebei,Shijianzhuang 050051,China
【Abstract】 Objective To detect the expression of proliferating cell nuclear antigen (PCNA ) in the human clear cell renal cell carcinoma(RCCC) tissues in order to explore the correlations between the antigen expression and RCCC.Methods The samples were divided into three groups: RCCC group, paracancerous tissue group and normal tissue group. All the samples were fixed in formalin for 48h, embedded in paraffin wax, sectioned to 5μm thick slice, and observed by immunohistochemical method. Results PCNA immunohistochemical staining showed that the expression of PCNA was weakly positive in the nucleus of the normal renal tissue. The expression was slightly higher in paracancerous tissue than that in normal renal tissue. The expression was significantly increased in RCCC tissue ,which was significantly higher than that in paracancerous tissue (P<0.01), but there were no significant differences among 4 stages (P>0.05). Conclusion the expression of PCNA in RCCC tissue is higher than that in para-cancerous tissue, which is the lowest in normal renal tissue. It may be one of the important mechanisms in tumor formation.
【Key words】 human renal clear cell carcinoma; proliferating cell nuclear antigen医.学全.在.线网站www.lindalemus.com
细胞增殖与凋亡是肿瘤形成的重要理论基础，是组织正常生长，行使功能的两个基本要素。二者互相制约，处于动态平衡之中，组织细胞受损或增殖过度时，通过凋亡清除受损及异常过多的细胞，防止组织进一步损伤，而凋亡或其他原因丢失的死亡细胞则由增殖来补充，以保持正常的细胞数目及体积。这些精细调节的失衡会导致疾病的发生[1]。组织细胞凋亡减少增殖过度就会导致肿瘤的发生，细胞增殖的研究是肿瘤形成探讨的重要方面。
1 材料与方法
1.1 材料 标本均来自2006年1月至2008年1月石家庄市第二医院和解放军260医院泌尿外科，其中肾透明细胞癌30例(Ⅰ期7例,Ⅱ期10例,Ⅲ期8例,Ⅳ期5例)、癌旁组织(距肿瘤组织边缘2.0 cm以上[2])30例(Ⅰ期7例,Ⅱ期10例,Ⅲ期8例,Ⅳ期5例)、意外伤亡正常肾组织10例(癌旁肾组织，和肾透明细胞癌取自同一患者)。肾透明细胞癌及癌旁组织标本来源的患者60例，男40例，女20例;年龄52～78岁，平均年龄64岁。正常肾组织标本来源的意外伤害者7例，男5例，女2例;年龄19～48岁，平均年龄35岁。
