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医学论文范文:大鼠股骨骨不连模型不同位点骨形态发生蛋白2基因的表达
【摘要】 目的： 观察大鼠股骨骨不连模型修复过程中骨形态发生蛋白2(bone morphogenetic protein2,BMP2)在各个时间段的表达情况,初步探讨单一生长因子对骨不连修复的影响。方法： SD大鼠分别于取材前35天、28天、21天、14天、7天、3天和1天建造股骨骨不连模型,同一天处死大鼠取材,RTPCR技术检测各个样本的BMP2基因的表达情况。结果： 在大鼠股骨骨不连修复过程的不同时间,BMP2基因表达在中心区和外周区存在差异,中心区BMP2基因表达滞后,表达的增幅及峰值低于边缘区。结论： 大鼠股骨骨不连中心区在修复早期BMP2基因的低表达及表达时间滞后可能是其骨不连发生的原因之一。
【关键词】 骨不连; 骨形态发生蛋白2; 大鼠
Expression of BMP2 gene in different region of rat’s femoral nonunion model
LI Yang1, QIAN Dong1, MA Peng2, LIU Xiaoping1, CUI Xuewen1, XU Xiaofeng1
(1.Department of Orthopedics, the Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001; 2.Department of Anesthesiology, the Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001,China)
[Abstract] Objective: To observe the genic dynamic expression of bone morphogenetic protein2(BMP2) during restoration of rat femoral nonunion and to discuss its influence to the restoration of rat femoral nonunion. Methods： The models of femoral nonunion of SD rats were established in day 1,3,7,14,21,28,35 before we extracted the specimens, and then the genic expression of BMP2 in different repairing stage of nonunion models were detected by RTPCT technique. Results： In different stages of femoral nonunion, the genic expression of BMP2 between central zone and outer zone also varied, their expression increment and the peak level were significantly lower in the central zone than those in the outer zone,and we can find the lag in the genic expression of BMP2 in the central zone. Conclusion： Lower value and the lag in genic expression of BMP2 in the central zone during the early stage may be an important reason of nonunion occurring in rat femur医.学全.在.线网站www.lindalemus.com.
[Key words] nonunion; bone morphogenetic protein2; rat
导致骨缺损、骨不连的原因仍不完全明了。在缺损区域促进骨形成是骨不连治疗的重要理念,在能够促使骨形成的因素中,最引人注目的是细胞因子的诱导和促进作用,其中成骨作用最为突出的是骨形态发生蛋白2(bone morphogenetic protein2,BMP2)。BMP2是最主要的调节骨形成的信号蛋白,BMP2基因的表达在骨的发生、诱导、修复和骨量保持方面发挥着重要而关键的作用,其表达水平的高低直接影响间充质骨细胞分化和骨量产生水平,而BMP2基因在骨不连中表达的相关报道较少。本实验通过建立大鼠股骨骨不连模型探讨骨不连修复过程中BMP2基因表达在时相和位相上差异,以初步了解BMP2基因在骨不连修复过程中的表达情况,探讨BMP2对骨不连修复可能产生的影响。
1 材料和方法
