 |
 |
综上所述，本研究从细胞水平初步探讨了大黄素对模拟冷缺血再灌注后肝细胞内钙离子浓度及凋亡的影响，提示大黄素有可能通过抑制细胞内钙超载及细胞凋亡，从而减轻肝细胞冷缺血再灌注损伤，为其在临床肝移植中的应用提供一定的实验依据。
【参考文献】
[1]YING D, KAM MK. Effects of emodin treatment on mitochondrial ATP generation capacity and antioxidant components as well as susceptibility to ischemia reperfusion injury in rat hearts: Single versus multiple doses and gender difference [J]. Life Sci, 2005, 77(22):27702782.
[2]YING D, KAM MK. Effects of pharmacological preconditioning by emodin/oleanolic acid treatment and/or ischemic preconditioning on mitochondrial antioxidant components as well as the susceptibility to ischemiareperfusion injury in rat hearts [J]. Mol Cell Biochem, 2006, 288(12):135142.
[3]刘影，孙宏丽，单宏丽，等. 大黄素对豚鼠单个心室肌细胞胞浆游离钙浓度的影响 [J].哈尔滨医科大学学报, 2003, 37(2):122124.
[4]刘影，单宏丽，孙宏丽，等. 大黄素对豚鼠单个心室肌细胞胞浆游离钙浓度及L2型钙电流的影响 [J]. 药学学报, 2004, 39(1):58医.学全.在.线网站www.lindalemus.com.
[5]祁翔，吕毅，刘昌，等. 大黄素对肝细胞模拟冷缺血再灌注损伤的保护作用及机制 [J]. 第四军医大学学报, 2008, 29(15):14171419.
[6]钟理，宋治远. 模拟缺血再灌注诱导原代培养乳鼠窦房结细胞凋亡的研究 [J]. 第三军医大学学报, 2001, 23(1):5961.
[7]ROBIN E, GUZY RD, LOOR G, et al. Oxidant stress during simulated ischemia primes cardiomyocytes for cell death during reperfusion [J]. J Biol Chem, 2007, 282(26):1913319143.
[8]LOUCH WE, FERRIER GR, HOWLETT SE. Attentuation of cardiac stunning by losartan in a cellular model of ischemia and reperfusion is accompanied by increased sarcoplasmic reticulum Ca2+ stores and prevention of cytosolic Ca2+ elevation [J]. J Pharmacol Exp Ther, 2005, 312(1):238247.
[9]WU T, DING XS, WANG W, et al. MCI186 (3Methyl1phenyl2pyrazolin5one) attenuated simulated ischemia/reperfusion injury in cultured rat hippocampal cells [J]. Biol Pharm Bull, 2006, 29(8): 16131617.
[10]BIELAWSKA AE, SHAPIRO JP, JIANG L, et al. Ceramide is involved in triggering of cardiomyocyte apoptosis induced by ischemia and reperfusion [J]. Am J Pathol, 1997, 151(5):12571263.
[11]QIN Y, TERRY L, VANDEN H, et al. Caspasedependent cytochrome c release and cell death in chick cardiomyocytes after simulated ischemiareperfusion [J]. Am J Physiol Heart Circ Physiol, 2004, 286(6):22802286.
[12]WU YX, TU X, LIN GS, et al. Emodinmediated protection from acute myocardial infarction via inhibition of inflammation and apoptosis in local ischemic myocardium [J]. Life Sci, 2007, 81(17):13321338.
[13]SHIEH DE, CHEN YY, YEN MH, et al. Emodininduced apoptosis through p53dependent pathway in human hepatoma cell [J]. Life Sci, 2004, 74(18):22792291.
