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医学论文范文:雌激素及拮抗剂三苯氧胺对子宫内膜癌细胞生长的影响
【摘要】 目的 研究雌激素及拮抗剂三苯氧胺对子宫内膜癌细胞HEC1B生长的影响。方法 体外培养中分化子宫内膜癌细胞HEC1B，按实验目的分别加入17β雌二醇(E2，10-8M)、三苯氧胺(TAM，10-7 M)刺激24、48、72、96 h后，采用四甲基偶氮唑蓝(MTT)比色方法比较各个条件下的细胞数目。用流式细胞术方法检测加入E2、TAM后的细胞周期变化，比较各个条件下的S期细胞比率(SPF)。结果 MTT结果：E2、TAM在作用72 h和96 h后，可显著刺激HEC1B细胞生长(P<0.05)。HEC1B细胞加入E2刺激后，SPF在加药72 h和96 h均增高。TAM作用细胞后，SPF值在药物作用48 h起均高于对照组。结论 雌激素、三苯氧胺均对子宫内膜癌HEC1B细胞有促生长作用。
【关键词】 子宫内膜肿瘤;雌激素;三苯氧胺;细胞生长
The effect of estrogen and tamoxifen on the growth of endometrial carcinoma cell LI Li^,LIAO Qinping,YU Li,et al.^ Department of Obstetrics and Gynecology,Ditan Hospital,Beijing 100015,China
【Abstract 】 Objective To investigate the effect of estrogen and tamoxifen on the growth of endometrial carcinoma cell line-HEC1B. Methods The endometrial cancer cell lineHEC1B was cultured in vitro. The cells were stimulated with 17βestrodiol(10-8M)and tamoxifen (10-7M)for 24 h,48 h,72 h,96 h respectively. The proliferation capacity of the cells was evaluated by means of mircocutrue tetrazolium assay (MTT) and the changes of cell cycle after E2 and TAM introduction were detected by flow cytometry (FCM).The cells percentage in S phase(SPF)in different conditions was compared.Results Both 17βestrodiol and tamoxifen could significantly stimulate the growth of HEC1B cell 72h and 96h after E2,TAM introduction. HEC1B cells could increase SPF 72h and 96h after E2 stimulation. The SPF of HEC1B cell 48h,72h,96h after TAM introduction was higher than that of control group.Conclusion Both estrogen and tamoxifen can stimulate the growth of HEC1B cell line.
【Key words】 endometrial carcinoma;estrogen;tamoxifen;cell growth
子宫内膜癌是女性生殖道最常见的三大恶性肿瘤之一，近年来其发病率呈上升趋势，占女性生殖系统恶性肿瘤的20%～30%[1]。目前普遍认为子宫内膜癌是激素依赖性肿瘤之一。其发生、发展与多种激素及其拮抗剂、受体密切相关。本实验研究雌激素及其拮抗剂三苯氧胺(TAM)对子宫内膜癌细胞生长的影响医.学.全.在.线www.lindalemus.com。
1 材料与方法
1.1 实验材料 选用中分化子宫内膜癌细胞系HEC1B，由北京大学第一医院妇产科研究室传代。
