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医学论文范文:6-OHDA诱导大鼠不同脑区星形胶质细胞反应差异的研究
【摘要】 目的：观察大鼠不同脑区中的星形胶质细胞对6-羟多巴(6-OHDA)局部注射反应的差异。方法：将6-OHDA局部注入大鼠脑内黑质致密部(SNc)、纹状体(CPu)、腹侧被盖区(VTA)和额叶联合皮层(FrA)，用免疫组织化学方法观察这些脑区中星形胶质细胞的变化。结果：6-OHDA注入1 d后各脑区星形胶质细胞反应较轻，7 d后反应明显，并持续至14 d;各组相比较以SNc组和VTA组反应明显，而CPu组和FrA组反应较轻。结论:大鼠不同脑区中的星形胶质细胞对6-OHDA局部注射后的反应存在差异。
【关键词】 星形胶质细胞;6-羟多巴;黑质致密部;纹状体;腹侧被盖区;额叶联合皮层;大鼠
The reactions of astrocytes in different brain regions in rat model induced by 6-OHDA
DONG Yulin, ZHANG Zhijun, NI Hengjian (Department of Human Anatomy, Medical School, Institute of Neurobiology, Nantong University; Jiangsu Key Laboratory of Neuroregeneration, Nantong 226001)
[Abstract] Objective:To observe the reactions of astrocytes in different brain regions in rat model induced by 6-OHDA. Methods:The reactive astrocytes in substantia nigra compact part(SNc), caudate putamen(CPu), ventral tegmental area(VTA)and frontal association cortex(FrA) induced by 6-OHDA were detected by immunohistochemical staining. Results:The reactions of astrocytes were hardly detected on day1 after injecting 6-OHDA. From day 7 on wards the reactions were significant. We also found that the reactions of astrocytes in SNc and VTA were more remarkable than that in CPu and FrA. Conclusion:The reactions of astrocytes in some related brain regions in rat model induced by 6-OHDA were different医学 全在.线提供www.lindalemus.com.
[Key words] Astrocyte;6-OHDA;Substantia nigra compact part;Caudate putamen;Ventral tegmental area;Frontal association cortex;Rat6-羟多巴(6-OHDA)是目前建立偏侧帕金森病(Parkinson’s disease，PD)
动物模型常用的神经毒剂，能选择性损伤单胺能神经元[1]。将其注入黑质致密部(SNc)、纹状体(CPu)或前脑内侧束时，会引起黑质DA能神经元的大量退变死亡及纹状体内的DA水平下降。在6-OHDA诱导的PD模型及PD患者脑中都发现CPu和SNc脑区出现明显的胶质细胞反应。将6-OHDA局部注入腹侧被盖区(VTA)或额叶联合皮层(FrA)能否同样诱导其中DA能神经元胞体及末梢的退变死亡，以及能否激活其内的星形胶质细胞?目前未见详细的研究报道。本实验将6-OHDA局部注入大鼠SNc、CPu、VTA和FrA脑区，观察这些脑区中星形胶质细胞反应的情况，借以探讨不同脑区中的星形胶质细胞对6-OHDA反应的差异性，为进一步研究星形胶质细胞参与DA能神经元及其末梢选择性损伤的机制及临床治疗PD等神经退行性疾病提供一定的实验依据。
1 材料与方法
1.1 实验动物和主要试剂 成年雄性SD大鼠48只，体重220 g左右(南通大学实验动物中心提供)。6-OHDA(Sigma公司)，小鼠抗GFAP(Chemicon公司)，HRP标记的山羊抗小鼠抗体(Chemicon公司)，二氨基联苯胺(DAB)(Sigma公司)。
