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医学免费论文:鼻息肉及下鼻甲黏膜成纤维细胞中组胺受体的表达
【摘要】 ［目的］了解鼻息肉成纤维细胞中组胺受体及其亚型的存在与否，比较鼻息肉与下鼻甲黏膜成纤维细胞中组胺受体的表达程度.［方法］选择5例鼻息肉组织，以同侧下鼻甲黏膜为对照.经鼻内窥镜手术切除鼻息肉及部分下鼻甲黏膜,培养成纤维细胞.采用RTPCR方法观察鼻息肉成纤维细胞中的组胺受体及其亚型，用实时PCR方法分别比较鼻息肉与下鼻甲黏膜成纤维细胞中组胺受体mRNA的表达情况；用流式细胞仪测定鼻息肉及下鼻甲黏膜成纤维细胞中组胺受体蛋白的表达.［结果］鼻息肉成纤维细胞中可见1型及2型组胺受体存在，其表达程度低于下鼻甲黏膜成纤维细胞，鼻息肉中仅可见少量的组胺受体蛋白表达.［结论］鼻息肉成纤维细胞中只有少量的1型及2型组胺受体的表达.
【关键词】 鼻息肉;受体,组胺;纤维母细胞
Expression of histamine receptor in fibroblast of nasal polyp and nasal turbinate mucosa
CUI Zhezhu, XU Yingji, ZHOU Haihui, CUI Chunlian, JIN Shunji^
(Department of Otorhinolaryngoloyg, Affiliated Hospital of Yanbian University, Yanji 133000, Jilin, China)
ABSTRACT:OBJECTIVETo investigate whether the histamine receptors and its subtypes were in the fibroblast of nasal polyp, and then to compare the degree of expression in fibroblasts between nasal polyp and inferior turbinate mucosa.METHODS Each of five tissues of nasal polyp and nasal inferior turbinate mucosa as controls were taken from inferior turbinate during nasal surgery, and the fibroblasts were cultured from each mucosa. The subtypes of histamine receptor in the fibroblasts were identified by RTPCR, the expression level of histamine receptors mRNA were by real time PCR, respectively, and the protein expression of histamine receptors were evaluated in nonstimulated condition by flow cytometry.RESULTSHistamine receptor 1 and 2 were identified in the fibroblasts of nasal polyp, and its expression level was lower in the fibroblasts of nasal polyp than that of inferior turbinate mucosa, and a little of histamine receptors protein was expressed in nasal polyp.CONCLUSIONSThere is a little of expression of histamine receptor 1 and 2 in the fibroblasts of nasal polyp医.学.全.在.线www.lindalemus.com.
Key words:nasal polyps;receptors, histamine;fibroblast
