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医学免费论文:异常黑胆质证载体大鼠模型的建立及其免疫、内分泌紊乱状态的研究
【摘要】 目的建立维吾尔医异常黑胆质证载体大鼠模型，并探讨其免疫、内分泌的紊乱状态。方法雄性Wistar大鼠20只, 稳定饲养3 d后随机分为正常对照组(10只)和模型组(10只),其中模型组根据维吾尔医学理论，采用干寒饲养环境、干寒属性的饲料、慢性间断足底电刺激、强迫游泳、制动等多因素复合作用3周，建立维吾尔医异常黑胆质证载体大鼠模型，动态观察模型组大鼠一般状态、体重、饮食量、饮水量和舌苔的变化;检测外周血白细胞介素1β(IL1β)、白细胞介素6(IL6)、肿瘤坏死因子α(TNFα)、促肾上腺皮质激素(ACTH)和皮质醇(CORT)等免疫、内分泌指标的变化。结果与正常对照组相比，模型组动物的一般状态与异常黑胆质证候特征相一致。第11天至第21天体重增长速度慢，自第7天至第21天饮食量、饮水量增多，差异有统计学意义(P<0.01)。与正常对照组相比，模型组动物血清ACTH、CORT、IL1β水平均降低，差异有统计学意义(P<0.05~0.01),IL6水平降低，但差异无统计学意义(P>0.05),TNFα水平升高,差异有统计学意义(P<0.05)。结论异常黑胆质证载体大鼠模型的一般状态、体重、饮食量、饮水量的变化与临床患者以及小鼠动物模型中异常黑胆质证相似，并且其免疫、内分泌处于紊乱状态。
【关键词】 异常黑胆质; 维吾尔医学; 动物模型
Establishment of abnormal savda syndrome rat model and study on its disordered immuneendocrine network
ZHANG Youhui, Halmurat·Upur, Yusup·Tursun, et al医.学.全.在.线www.lindalemus.com
(Department of Biochemistry, Preclinical Medicine College, Xinjiang Medical University, Urumqi 830011, China)
Abstract: ObjectiveTo establish a rat model of abnormal savda syndrome in Uygur medicine and investigate its disordered immuneendocrine network. MethodsMale Wistar rats (n=20)， were randomly divided into control group (n=10) and model group (n=10) after stable feeding 3 d. According to the theory of Uygur medicine, model group rats were fed with coldnatured diet under cool dry environment, stimulated with chronics and interrupted electric foot shocks, enforced swimming and controlled movement to establish abnormal savda syndrome rat model for three weeks, rats′ behavior, body weight, daily food intake, daily water intake, state of tongue were observed. The concentrations of immuneendocrine related indexes including Interleukin1β (IL1β), Interleukin6 (IL6), Tumor necrosis factorα (TNFα), Adrenocorticotropic Hormone (ACTH) and Cortisol (CORT) in serum were measured by enzyme linked immunosorbent assay (ELISA) method. ResultsCompared with the control group, the model group developed abnormal savda syndrome like slow weight gaining, more diet, more drink. Compared with the control group, the serum level of ACTH， CORT， IL1β in model group decreased，and the differences had statistical significance (P<0.05~ 0.01); the serum level of IL6 in model group decreased，but the differences had no statistical significance (P>0.05); the serum level of TNFα increased, and the differences had statistical significance (P<0.05). ConclusionThe changes of body weight, daily food intake, daily water intake of abnormal savda syndrome rat model are similar to the feature of human abnormal savda syndrome and the abnormal savda syndrome model of ICR mice. Abnormal savda syndrome rat model developes disordered immuneendocrine network.
Key words: abnormal savda; Uygur medicine; animal model
维吾尔医学认为异常黑胆质是在各种内外因素的作用下，体液质平衡紊乱，体液质被燃烧，继而沉淀的最终病理产物或表现形式。长期受到慢性刺激、进食干寒属性的饲料、处于干寒的环境是异常黑胆质证发生的主要起因[1-2]。随着维吾尔医学的深入研究,需要建立具有维吾尔医学证候特征的大鼠模型,以便运用现代科学手段研究维吾尔医学。本研究以维吾尔医学理论为指导，采用干寒饲养环境、干寒属性的饲料、慢性间断足底电刺激、强迫游泳和制动等多因素复合作用3周，建立维吾尔医异常黑胆质证载体大鼠模型，并检测其外周血白细胞介素1β(IL1β)、白细胞介素6(IL6)、肿瘤坏死因子α(TNFα)、促肾上腺皮质激素(ACTH)和皮质醇(CORT)等免疫、内分泌指标的变化，探讨其免疫、内分泌的紊乱状态。
1 材料与方法
