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医学免费论文:快速筛选调节肝脂酶活性中药成分方法的研究
【摘要】 目的 研究快速筛选调节肝脂酶活性中药成分的方法。方法 利用大鼠肝组织匀浆和比色法检测肝脂酶(HL)活性，观察不同实验条件及中药成分对 HL活性的影响。结果 正常大鼠肝组织匀浆具有明显的HL酶促动力学特性，其HL脂解活性有明显Ca2+依赖性，受钙调蛋白拮抗剂盐酸异丙嗪抑制，符合纯化HL的酶促动力学特性;筛选得到齐墩果酸和人参皂苷Rb2 2个增强肝脂酶活性成分。结论 首次用大鼠肝组织匀浆和脂酶比色测定法建立了一种简便易行、快捷的调节肝脂酶活性中药有效成分筛选方法，可用于影响肝脂酶活性的中药调脂活性成分的筛选，并从有效方药组分中筛选得到有增强HL活性的组分。
【关键词】 肝脂酶;快速筛选;大鼠肝匀浆;复方贞术调脂方;肝脂酶激活剂
Rapid screening of active ingredients from Chinese medicine regulating hepatic lipase activitiesBEI Weijian，GUO Jiao，HU Yinming，HUANG Lihua，HU Xuguang，CAO Yang医.学全.在.线网站www.lindalemus.com
(Key Research Center of Liver Regulating for Hyperlipidemia，SATCM/ Class Ⅲ Laboratory of Lipid Metabolism， SATCM/ Academy of Tradational Chinese Medicine,Guangdong Pharmaceutical College，Guangzhou,Guangdong 510006,China)Abstract:Objective To study the new approach for rapid screening of active ingredients from Chinese medicine regulating hepatic lipase activities.Methods Rat liver homogenate and colorimetric assay of hepatic lipase (HL) activities were used to investigate the effects of different components of Chinese medicine on hepatic lipase activities.Result It was found that liver homogenate of SD rats demonstrated significant enzyme kinetic properties of HL. Its lipolytic activities was significantly Ca2+dependent,and inhibited by calmodulin antagonist promethazine hydrochloride,consistent with the enzymatic kinetic properties of purified HL reported in the literature. Oleanolic acid and ginsenosides Rb2 was screened out as the activators of HL.Conclusion The method for rapid screening of the active ingredient of Chinese medicine regulating HL activities by using rat liver homogenate in combination with colorimetric assay of HL activities was developed,which was simple and reliable,and could be used in .rapid screening of lipidlowering ingredients in traditional Chinese medicine having effects on HL activities,and screening of active ingredients that might increase HL activities from effective recipes of traditional Chinese medicine.
Key words:hepatic lipase (HL) ; rapid screening; rat liver homogenate; Fufang Zhenzhu Tiaozhi Fang(FTZ) recipe;hepatic lipas activator
肝脂酶(hepatic lipase,HL)是重要的脂代谢酶，其合成、分布、活性的变化和高脂血症、脂肪肝、动脉粥样硬化等密切相关。肝组织和血浆中HL酶活性的降低，可引起高脂血症[1-3]，并在脂肪肝发病中起重要作用[4，5]。
复方贞术调脂方(简称FTZ)由女贞子、丹参、三七、黄连等中药组成，含有齐墩果酸、三七皂苷、黄连素、丹参素等活性成分，在临床应用和实验研究中发现该方不仅具有明显的降低总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDLC)及升高高密度脂蛋白胆固醇(HDLC)的作用,并能升高血浆HL活性、抑制动脉粥样硬化(AS) 形成、抑制脂肪肝形成等作用[6，7]，还能使饮食性高脂血症模型大鼠降低的肝组织和血浆中HL的活性恢复至正常水平[7]。
为了研究中药降血脂的物质基础和筛选新药，本研究以HL为靶点，以FTZ组分为调节HL活性成分的筛选对象，研究建立一种采用大鼠肝匀浆比色法测定肝脂酶活性，快速筛选调节HL活性中药有效成分的方法，现报道如下。
