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医学免费论文:布洛芬聚乙二醇b聚L乳酸电纺超细纤维毡的药物释放
【摘要】 目的 通过静电纺丝制备同时包载有布洛芬的聚乙二醇b聚L乳酸纤维毡,并在纤维中添加月桂酸,考察月桂酸是否对纤维中药物的释放行为有影响。方法 以聚乙二醇嵌段聚乳酸为载体材料,有机溶液高压静电法纺丝法制得同时包载布洛芬和月桂酸的纤维毡,采用ESEM、WAXD和DSC对其进行表征研究,采用HPLC法测定布洛芬在含有蛋白酶K的磷酸缓冲盐溶液中的释放行为。结果 ESEM结果显示得到添加有月桂酸的聚乙二醇聚乳酸纤维药物释放体系,WAXD扫描显示纤维表面无药物结晶析出,说明其对布洛芬和月桂酸完全包封,药物体外释放表明添加月桂酸后,药物释放速率加快明显。结论 在聚乙二醇聚乳酸超细纤维添加月桂酸可加快纤维中布洛芬的释放，蛋白酶K的降解也可加快布洛芬的释放。
【关键词】 超细纤维; 布洛芬; 静电纺丝; 聚乳酸; 药物释放; 月桂酸医.学全.在.线网站www.lindalemus.com
Drug release from PEGPLLA electrospun fibrous mat with ibuprofen entrapped
WANG Hao1,2,CUI Yong3,JIAO Zixue2,CHEN Xuesi2,DENG Yingjie1
(1.School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China;2.Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun Jinlin 130022,China;3.School of Life Science and Pharmacology,Shenyang Pharmaceutical University,Shenyang 110016,China)
【Abstract】 Objective To prepare poly ethylene glycol blocked poly lactic acid ultrafine fibers with ibuprofen entrapped by electrospinning and add lauric acid into fibers to investigate whether or not lauric acid affects the in vitro release of ibuprofen.Methods Fiber mats were prepared with both ibuprofen and lauric acid added by organic solvent highvoltage electrospinning technique by using poly ethylene glycol blocked poly lactic acid as drug loading material.The characterization of fiber mats was researched by ESEM,WAXD and DSC.The release behavior of ibuprofen in PBS with proteinase K added was investigated by HPLC.Results PEGPLLA fiber drug delivery system with lauric acid added was obtained,WAXD scanning demonstrated that no drug crystal was decomposed and all compounds were perfectly entrapped.After lauric acid addition,ibuprofen released faster.Conclusion Addition of lauric acid could facilitate ibuprofen release from PEGPLLA fibers.Moreover,proteinase K could facilitate ibuprofen release from PEGPLLA fibers.
【Key words】 ultrafine fiber;ibuprofen;electrospinning;poly lactic acid;drug release;lauric acid
静电纺丝技术是一种制备微米及纳米尺度纤维的新方法,近些年有不少文献采用此方法将药物包载在医用高分子材料中,进行了一些前瞻性的研究[15]。载药纤维制备的大体过程包括将药物溶于或分散于待纺丝的高分子聚合物有机溶液中或熔体中,将聚合物溶液或熔体带上103～104 V高压静电,在强静电场作用下带电的聚合物溶液滴在电场的作用力下克服液体表面张力于毛细管的末端形成Taylor锥体,并以静电力为牵引力来形成喷射流进行纺丝,在此过程中,由于溶剂的快速挥发,药物将以极小的颗粒或分子(离子)状态存在于聚合物纤维中最终在接收装置上获取一种新型的药物释放系统——超细纤维[6，7],纺丝装置及原理如图1所示。
能够进行静电纺丝的高分子材料有很多,聚L乳酸是脂肪族聚酯中在人体中生物相容性和生物可降解性最好的材料之一,并且由于其亲脂的化学结构,其可对许多在水中溶解度低的化合物进行包裹,是近些年研究应用较多的一类高分子药物载体材料[8，9]。将PLLA用适当分子量的聚乙二醇(PEG)修饰后可使高分子呈现一定的亲水性,改善其在体内的应用[10]。本实验拟通过对溶解在有机溶剂中的聚乳酸进行静电纺丝制备得到超细生物可降解纤维。
