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医学免费论文:基质金属蛋白酶9在哮喘大鼠中性粒细胞中的表达
【摘要】 目的:观察基质金属蛋白酶9(MMP-9)在哮喘大鼠血中性粒细胞(PMN)中的表达，探讨PMN能否通过合成MMP-9参与哮喘的发病。方法：健康雄性SD大鼠30只，随机分成哮喘组（A组)、布地奈德治疗组（B组)和正常对照组（C组)，用卵蛋白（OVA)致敏和激发的方法建立哮喘模型。分离纯化血PMN，免疫细胞化学法检测MMP-9的表达水平，ELISA法检测血清基质金属蛋白酶抑制剂-1（TIMP-1)的浓度。结果：A组（0.196±0.011，OD值)PMN MMP-9的表达水平显著高于C组（0.100±0.010，OD值)（P <0.01)，B组（0.135±0.008，OD值)PMN MMP-9的表达水平显著低于A组但高于C组（均P <0.01)。A组血清TIMP-1为(34.96±4.54 )ng/mL，表达水平显著高于C组的(26.14±3.43) ng/mL（P <0.01)，B组血清TIMP-1的表达水平显著低于A组但高于C组（P <0.05或P <0.01)。MMP-9与TIMP-1的表达水平呈显著正相关(n =29，r=0.713，P <0.01)。结论：哮喘大鼠PMN合成MMP-9的功能增加，PMN可能部分通过增加合成MMP-9参与哮喘的发病，这种功能可以部分被布地奈德所抑制。
【关键词】 哮喘；MMP-9；TIMP-1；PMN；大鼠
Expressions of matrix metalloproteinase-9 in blood polymorphonuclear leucocyte of asthmatic rats JING Wei-ying^，YE Hui，TONG Xia-sheng，WANG En-zhi，GUO Hai-yuan，RUAN Zheng-ying，JING Xiao-hong.^Department of Pediatrics，Taizhou Integrated Traditional and Western Medicine Hospital，Taizhou， 317523医.学全.在.线www.lindalemus.com
Abstract: Objective: To observe the expressions of matrix metalloproteinase-9 (MMP-9) in blood polymorphonuclear leukocyte (PMN) of asthmatic rats. And to approach whether PMN could participate in asthma pathogenesis through synthesizing MMP-9. Methods ：Thirty rats were randomly divided into three groups on average， including asthmatic group (group A)， budesonide treated group (group B) and control group (group C). Blood PMN were isolated and purified. The expressions of MMP-9 were detected with immunocytochemical method in blood PMN. The concentration of matrix metallo-proteinase inhibitor-1 (TIMP-1) was detected with ELISA. Results ：The level of MMP-9 in group A （0.196±0.011，Optical density) were significantly higher than those in group C （0.100±0.010)(P <0.01). Levels of MMP-9 in group B （0.135±0.008) were significantly lower than those in group A， but higher than those in group C (all P <0.01). Levels of TIMP-1 in group A （34.96±4.54 ng/mL) were significantly higher than those in group C（26.14±3.43 ng/mL)(P <0.01). Levels of TIMP-1 in group B （29.58±2.41 ng/mL) were significantly lower than those in group A， but higher than those in group C （P <0.05 or P <0.01). There was significant positive correlation between the level of MMP-9 and TIMP-1(n =29，r =0.713，P <0.01). Conclusion：Levels of MMP-9 synthesized by PMN increase in asthma rats. PMN may be implicated with asthma pathogenesis via increasing synthesis of MMP-9，but this function of PMN can be partly inhibited by budesonide.
Key words: asthma；matrix metalloproteinase-9；TIMP-1；polymorphonuclear leukocyte；rat
支气管哮喘是一种由多种炎性细胞和细胞组分参与的气道慢性炎症，气道重构和气道高反应性是其特征性病理改变。基质金属蛋白酶9(matrix metalloproteinase-9，MMP-9)及其抑制剂——基质金属蛋白酶抑制剂-1（TIMP-1)，在哮喘的气道炎症和气道重构中起着重要作用[1]。最近研究发现，中性粒细胞（PMN)能释放多种酶、炎症递质和细胞因子参与哮喘的炎症过程[2]，但PMN能否通过MMPs/TIMPs体系参与哮喘的发病，目前知之甚少。本实验通过检测哮喘大鼠血PMN MMP-9和血清TIMP-1的表达水平，探讨它们在哮喘中的可能作用机制。
