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医学免费论文:肺炎支原体肺炎患儿血清细胞因子与免疫球蛋白的检测及其临床意义
【摘要】 目的 探讨检测肺炎支原体肺炎(MPP)患儿血清细胞因子(IL6、IL8、TNFα)、免疫球蛋白(IgA、IgM、IgG)及补体(C3、C4)的临床意义。方法 60例MPP患儿(观察组)和60例健康儿童(正常对照组)为研究对象，采用放射免疫分析法测定IL6、IL12、TNFα，采用速率散射比浊法测定血清免疫球蛋白(Ig)和补体(C)。结果 观察组血清IL6、IL8的水平与正常对照组比较上升(P<0.05);而TNFα水平2组比较差异无统计学意义(P>0.05);观察组血清免疫球蛋白IgA、IgM、IgG水平、补体C水平与正常对照组比较上升(P<0.05)。结论 血清IL6、IL8、免疫球蛋白IgA、IgM、IgG，补体C3、4在MPP中起着重要作用，检测这些指标可以较准确地判定MMP患儿病情和预后，值得临床推广应用。
【关键词】 肺炎支原体肺炎;白介素6;白介素8;肿瘤坏死因子;免疫球蛋白Ig;补体C
Clinical significance of serum cytokines and immunoglobulin levels in children with mycoplasma pneumoniae pneumonia XIE Zhicai, LV Hui, ZHOU Shuming. Longgang Central Hospital, Guangdong, Shenzheng 518116,China
【Abstract】 Objective To investigate the clinical significance of detecting serum cytokines (IL6, IL8 and TNFα), immunoglobulins (IgA, IgM and IgG) and complements (C3 and C4) levels in children with mycoplasma pneumoniae pneumonia (MPP). Methods 60 children with MPP (observation group) and 60 healthy children (control group) were enrolled in the study. The serum levels of IL6, IL8 and TNFα were detected by ELISA method, the serum levels of immunoglobulins (IgA, IgM and IgG) and complements (C3 and C4) were determined by rate nephelometry method.Results The serum levels of IL6 and IL8 in observation group were significantly higher than those of control group (P<0.05),however there was no significant difference in TNFα levels between the two groups (P> 0.05). The serum levels of IgA, IgM，IgG，C3 and C4 in observation group were significantly higher than those of control group (P<0.05).Conclusion The serum levels of IL6 and IL8, immunoglobulins (IgA, IgM and IgG) and complements (C3 and C4) play an important role in MPP.The detection of these parameters can more accurately determine the disease condition and patients’prognosis,which is worthy to be popularized.
【Key words】 mycoplasma pneumoniae pneumonia;IL6; IL8; TNF;immunoglobulin; complement
肺炎支原体肺炎(MPP)又可称原发性非典型性肺炎，其病原体为肺炎支原体(MP)。MP广泛分布于自然界，是一种介于细菌和病毒之间的、能独立生活的最小微生物，是小儿呼吸道感染中较常见的病原体，是引起小儿下呼吸道感染的重要病原之一。已有研究认为，在MPP发病机制中，众多细胞因子都直接或间接地参与了免疫反应、炎性反应及免疫损伤[1]。认为内源性炎性介质白介素6(IL6)、IL8和肿瘤坏死因子α(TNFα)的大量释放，是引起继发性肺损伤的重要因素之一[2]。在MPP体液免疫方面，免疫球蛋白IgA、IgM、IgG、C3、C4与疾病的严重程度和病程长短也是目前人们研究的焦点。因此本文对MPP患儿血清炎性细胞因子IL6、IL12、TNFα和免疫球蛋白IgA、IgM、IgG、C3、C4水平进行研究，以探讨其在诊治小儿MPP中的价值，并提供理论依据医.学全.在.线网站www.lindalemus.com。
1 资料与方法
