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【关键词】 高脂血症;过氧化脂质;植物,药用
[摘要] [目的] 探讨黄芪对高脂血症小鼠血脂及脂质过氧化的影响. [方法] 取小鼠随机分组，除正常组外的其他组小鼠灌胃给予脂肪乳剂，建立高脂血症小鼠模型，分别灌胃给予不同剂量黄芪组小鼠18.0，9.0，4.5g/kg黄芪水提取物，连续4周，测定各组小鼠血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)及低密度脂蛋白胆固醇(LDL-C)含量，谷胱甘肽过氧化物酶(GSH-Px)和超氧化化物歧化酶(SOD)活性，脂质过氧化物(LPO)含量. [结果] 不同剂量黄芪组小鼠血清TC，TG，LDL-C含量明显下降，SOD，GSH-Px活性明显增强，血清LPO含量明显下降. [结论] 黄芪具有调节小鼠血脂代谢及增强机体抗脂质过氧化的作用.
[关键词] 高脂血症;过氧化脂质;植物，药用
Effects of Astragalus on serum lipids and lipoperoxidation in experimental hyper lipidemic mice
JIN Guang-xian 1 ，ZHANG Xue-wu 2^
(Department of Pathology and Forensic Medicine;Department of Biochemistry and Molecular Biology:Yanbian University College of Basic Medicine，Yanji133000，Jilin，China)
ABSTRACT:OBJECTIVE To investigate the effects of Astragalus on serum lipids and lipoperoxidation in experimental hyperlipidemic mice.METHODS The mice were randomly divided，and apart from the mice in normal group，all the mice in other groups were fed with lipid emulsion for4weeks.After the in experimental hyper lipidemic mice were succeed，different dosages of experimental control groups in which the mice were treated with Astragalus at dosages of18.0，9.0，4.5g/kg respectively.After4weeks，the TC，TG，HDL-c，LDL-c，SOD，GSH-Px and LPO in serum were measured.RESULTS Astragalus could significantly decrease the serum TC，TG，HDL-c in the experimental hyperlipidemic mice，and markedly increase the level of serum HDL-c.Mean level of serum LPO in the experimental groups treated by Astragalus at different dosages were much lower than that in hyper lipidema group，and the SOD and GSH-Px activities of serum in the group of Astragalus were much higher than those in hyper lipidema group.CONCLUSIONAstragalus can regulate lipid metabolism，enhance the antioxidation and reduce the lipid peroxidation in mice with hyperlipidemia医.学.全.在.线www.lindalemus.com.
Key words:hyperglycemia;lipid peroxides;plants，medicinal
黄芪具有补气升阳、固表止汗、脱疮生肌及利水消肿之功效，用于治疗表虚自汗、阳虚血脱及水肿血痹等症.本研究探讨了黄芪水提取物对高脂血症小鼠体内血脂及脂质过氧化的影响.
1 材料与方法
1.1 材料
1.1.1 实验动物 取昆明种小鼠，雌雄各半，体重为18～22g，由延边大学医学部实验动物科提供. 1.1.2 药物与试剂 丙基硫氧嘧啶为上海复兴朝辉药业有限公司产品;胆固醇为去氧胆酸钠;猪油为自制;实验所需其他试剂均为国产分析纯.GSH-Px，SOD，LPO试剂盒均为南京建成生物工程研究所产品.722型分光光度计为中国厦门分析仪器厂产品;7600-010型全自动生物化学检测仪为日本日立公司产品;低温高速离心机为美国Sigma公司产品. 1.2 方法
