1.BigbeeJW,et al . Effects of primary antiserum dilution on staining of antlgen richtissue with the peroxidase antiperoxidase technique. j Histochem Cytochem. 1977；25：433～447

2.BorrsmaDM. Preparation of HRP Iabelled antibodies。In Cuello AC(ed) lmmunohistochemistry, JohnWiley&,Sons, New York ：Bris－bane Toronto Singapore, 1983：87～100

3.ConchoroffNJ, et al . A monoclonal antibody reactive with 5—Boromo—2 deoxyuridine thatdoes not requireDNA denaturation。Cytometry,1985；6t506～512

4.CostaM, et al. Immunohistochemical localization of polypeptides in peripheralautononic nerves using whole mount preparation, Histo－chemistry, 1980；65：157～165

5.CummingR. Cyclic nucleotide losses during tissue processing for immunohistochemistry。j HistochemCotychem, 1980j28：54～55

6.GallJG, Paudue M. Formation and detection of RNA—DNa hybrid molecules incytological preparations. Proc Natu Acad Sci USA, 1969：63,378～383

7.Grzanwww.lindalemus.com/shiti/naR, Light microscopic immunocytochemistry with fixed unembedded tissue, InBullock GR&Petrusz P·(eds.)Techniques in immunocytochemistry. London：Academic Press inc, 1982；1：183～204

8.KohlerG, Milstein C. Continuous cultures of fused cells secreting antibody ofpredefined speciflcity, Nature,1975；256：495～497

9.ImagawaM, et al. Characteristic and evaluation of antibody horseradish peroxidaseconjugates prepared by using maleimide compound,glutaraldehyde and periodate. JBiochem, 1982 j 4：41～57

10.Li CY,et al. Use of azide and hydroRen peroxide as an inhibitor for endogenousperoxidase in the immunoperoxidase method , J Histochem Cytochem, 1987；35： 1457～1640

11.Matsuno K, et al. Splenic outer periarterial lymphoid sheath (PALS)：An immunoproliferative microen-vironment constituted by antigen laden marginal metallophils and ED3 positivemacrophages in the rat. Cell Tissue Res, 1989；257：459～470

12.NakanePK, Pierce GB. Enzyme labelled antibodies：Preparation and application for thelocalization of antigens。j Histochem Cytochem, 1966；14：929～931

13.NorrdenSV, Polak JM. Immunocytochemistry today techniques and practice, In PolakJM&Norrden SV(eds.) Immunocytdchemistry. London：Wright PSG Bristol, 1983：11～42

14. OiVT, Herzenberg LA. Imrnunoglobulin producing hybrid cell lines. In Mishell BBand Shiigi SM, (eds.) Selected methods in cellular immunology, San Francisco：Freeman WH and Company, 1980：351～375

15. SatoY, et al . The AMeX method. A simplified technique of tissue processing andparaffin embedding with improved preservation of antigens for immunostaining.Am J Pathol, 1986；125：431～435

16.Shi SR,et al . Antigen retrieval in forrnalin fixed,paraffin embedded tissue：An enhancement method forimmunohisotchemical staining based on microwave oven heating of tissue sections。j Histochem Cytochem, 1991；39：741～748

17.SternbergerLA, et al . The unlabelled antibody enzyme method of immunohistochemistry.Preparation and properties of soluble antigen –antigody complex (horseradishperoxidse antihorseradish peroxidase ) and its use in the identificationspirochetes, J Hsitochem Cytochem, 1970；18：315～333

18.Sternberger LA, Iwww.med126.commmunocytochemistry。New York：John Wiley&Sons, 1979

19.SternbergerLA, Conjugated antibody method. In ：Sternberger LA(ed) Immunocytochemistry。3rd edi—tion, New Yor ：John Wiley&Sons, 1986

20. VaccaLL. Double bridge technique of immunocytochemistry.In：Bullock GR&Petrusz P. (eds,)Tech. niques in Immunocytochemistry. London：Academic Press inc, 1982；1：155～182

21.Yasuda K, et al. Application of ultrasound for fixation：Combined use with microwave toenhance the effect of chemical fixation. Acta Histochem Cytochem, 1992；25： 237～244

22. ZhouDS, Komuro T. Interstitial cells associated with the deep muscular plexus ofthe guinea—pig small intestine, with special reference to the interstitialcells of Cajal. Cell Tissue Res, 1992；268： 205～216