| 公开(公告)号 | CN1560066A |
| 公开(公告)日 | 2005.01.05 |
| 申请(专利)号 | CN200410010714.7 |
| 申请日期 | 2004.03.03 |
| 专利名称 | 抗隐孢子虫重组免疫毒素蛋白及其制备工艺和医用用途 |
| 主分类号 | C07H21/04 |
| 分类号 | C07H21/04;C12N15/11;C12N15/09;C12N15/63;A61K38/16;A61P33/02 |
| 分案原申请号 | |
| 优先权 | |
| 申请(专利权)人 | 中国人民解放军军需大学 |
| 发明(设计)人 | 张西臣;李建华;蔡辉 |
| 地址 | 130062吉林省长春市西安大路175号 |
| 颁证日 | |
| 国际申请 | |
| 进入国家日期 | |
| 专利代理机构 | 吉林长春新纪元专利代理有限责任公司 |
| 代理人 | 陈宏伟 |
| 国省代码 | 吉林;22 |
| 主权项 | 抗隐孢子虫重组免疫毒素蛋白的基因序列如下：CAGGTGAAGCTGCAGGAGTCAGGACCTGGCCTGGTGGCACCCTCA45GlnValLysLeuGlnGluSerGlyProGlyLeuValAlaProSer151015CAGAGCCTGTCCATCACATGCACTGTCTCTGGGTTCTCATTATCC90GlnSerLeuSerIleThrCysThrValSerGlyPheSerLeuSer16202530AGATATAGTGTACACTGGGTTCGCCAGCCTCCAGGAAAGGGTCTG135ArgTyrSerValHisTrpValArgGlnProProGlyLysGlyLeu31354045GAGTGGCTGGGAATGATATGGGGTGGTGGAAGCACAGACTATAAT180GluTrpLeuGlyMetIleTrpGlyGlyGlySerThrAspTyrAsn46505560TCAGCTCTCAAATCCAGACTGAGCATCAGCAAGGACAACTCCAAG225SerAlaLeuLysSerArgLeuSerIleSerLysAspAsnSerLys61657075AGCCAAGTTTTCTTAAAAATGAACAGTCTGCAAACTGATGACACA270SerGlnValPheLeuLysMetAsnSerLeuGlnThrAspAspThr76808590GCCATGTACTACTGTGCCAGAAACCGGTATGGTAATGCTATGGAC315AlaMetTyrTyrCysAlaArgAsnArgTyrGlyAsnAlaMetAsp9195100105TACTGGGGCCAAGGCACCACGGTCACCGTCTCCTCAGGTGGAGGC360TyrTrpGlyGlnGlyThrThrValThrValSerSerGlyGlyGly106110115120GGTTCAGGCGGAGGTGGCTCTGGCGGTGGCGGATCGGACATCGAG405GlySerGlyGlyGlyGlySerGlyGlyGlyGlySerAspIleGlu121125130135CTCACTCAGTCTCCAGCCTCCCTATCTGTATCTGTGGGAGAAACT450LeuThrGlnSerProAlaSerLeuSerValSerValGlyGluThr136140145150GTCACCATCACATGTCGAGCAAGTGAGAATATTTACAGTAATTTA495ValThrIleThrCysArgAlaSerGlnAsnIleTyrSerAsnLeu151155160165GCATGGTATCAGCAGAAACAGGGAAAATCTCCTCAGCTCCTGGTC540AlaTrpTyrGlnGlnLysGlnGlyLysSerProGlnLeuLeuVal166170175180TATGCTGCAACAAACTTAGCAGATGGTGTGCCATCAAGGTTCAGT585TyrAlaAlaThrAsnLeuAlaAspGlyValProSerArgPheSer181185190195GGCAGTGGATCAGGCACACAGTATTCCCTCAAGATCAACAGCCTG630GlySerGlySerGlyThrGlnTyrSerLeuLysIleAsnSerLeu196200205210CAGTCTGAAGATTTTGGGAGTTATTACTGTCAACATTTTTGGGGT675GlnSerGluAspPheGlySerTyrTyrCysGlnHisPheTrpGly211215220225ACTCCGTACACGTTCGGAGGGGGGAGCAAGCTGGAGCTGAAACGG720ThrProTyrThrPheGlyGlyGlySerLysLeuGluLeuLysArg226230235240CATATGGCCGAAGAGGGCGGCAGCCTGGCCGCGCTGACCGCGCAC765HisMetAlaGluGluGlyGlySerLeuAlaAlaLeuThrAlaHis241245250255CAGGCTTGCCACCTGCCGCTGGAGACTTTCACCCGTCATCGCCAG810GlnAlaCysHisLeuProLeuGluThrPheThrArgHisArgGln256260265270CCGCGCGGCTGGGAACAACTGGAGCAGTGCGGCTATCCGGTGCAG855ProArgGlyTrpGluGlnLeuGluGlnCysGlyThrProValGln271275280285CGGCTGGTCGCCCTCTACCTGGCGGCGCGGCTGTCGTGGAACCAG900ArgLeuValAlaLeuTyrLeuAlaAlaArgLeuSerTrpAsnGln286290295300GTCGACCAGGTGATCCGCAACGCCCTGGCCAGCCCCGGCAGCGGC945ValAspGlnValIleArgAsnAlaLeuAlaSerProGlySerGly301305310315GGCGACCTGGGCGAAGCGATCCGCGAGCAGCCGGAGCAGGCCCTG990GlyAspLeuGlyGluAlaIleArgGluGlnProGluGlnAlaLeu316320325330GCCCTGACCCTGGCCGCCGCCGAGAGCGAGCGCTTCGTCCGGCAG1035AlaLeuThrLeuAlaAlaAlaGluSerGluArgPheValArgGln331335340345GGCACCGGCAACGACGAGGCCGGCGCGGCCAACGCCGACGTGGTG1080GlyThrGlyAsnAspGluAlaGlyAlaAlaAsnAlaAspValVal346350355360AGCCTGACCTGCCCGGTCGCCGCCGGTGAATGCGCGGGCCCGGCG1125SerLeuThrCysProValAlaAlaGlyGluCysAlaGlyProAla361365370375GACAGCGGCGACGCCCTGCTGGAGCGCAACTATCCCACTGGCGCG1170AspSerGlyAspAlaLeuLeuGluArgAsnTyrProThrGlyAla376380385390GAGTTCCTCGGCGACGGCGGCGACGTCAGCTTCAGCACCCGCGGC1215GluPheLeuGlyAspGlyGlyAspValSerPheSerThrArgGly391395400405ACGCAGAACTGGACGGTGGAGCGGCTGCTCCAGGCGCACCGCCAA1260ThrGluAsnTrpThrValGluArgLeuLeuGlnAlaHisArgGln406410415420CTGGAGGAGCGCGGCTATGTGTTCGTCGGCTACCACGGCACCTTC1305LeuGluGluArgGlyTyrValPheValGlyTyrHisGlyThrPhe42l425430435CTCGAAGCGGCGCAAAGCATCGTCTTCGGCGGGGTGCGCGCGCGC1350LeuGluAlaAlaGlnSerIleValPheGlyGlyValArgAlaArg436440445450AGCCAGGACCTCGACGCGATCTGGCGCGGTTTCTATATCGCCGGC1395SerGlnAspLeuAspAlaIleTrpArgGlyPheTyrIleAlaGly451455460465GATCCGGCGCTGGCCTACGGCTACGCCCAGGACCAGGAACCCGAC1440AspProAlaLeuAlaTyrGlyTyrAlaGlnAspGlnGluProAsp466470475480GCACGCGGCCGGATCCGCAACGGTGCCCTGCTGCGGGTCTATGTG1485AlaArgGlyArgIleArgAsnGlyAlaLeuLeuArgValTyrVal481485490495CCGCGCTCGAGCCTGCCGGGCTTCTACCGCACCAGCCTGACCCTG1530ProArgSerSerLeuProGlyPheTyrArgThrSerLeuThrLeu496500505510GCCGCGCCGGAGGCGGCGGGCGAGGTCGAACGGCTGATCGGCCAT1575AlaAlaProGluAlaAlaGlyGluValGluArgLeuIleGlyHis511515520525CCGCTGCCGCTGCGCCTGGACGCCATCACCGGCCCCGAGGAGGAA1620ProleuProLeuArgleuAspAlaIleThrGlyProGluGluGlu526530535540GGCGGGCGCCTGGAGACCATTCTCGGCTGGCCGCTGGCCGAGCGC1665GlyGlyArgLeuGluThrIleLeuGlyTrpProLeuAlaGluArg541545550555ACCGTGGTGATTCCCTCGGCGATCCCCACCGACCCGCGCAACGTC1710ThrValValIleProSerAlaIleProThrAspProArg |
| 摘要 | 本发明公开一种抗隐孢子虫重组免疫毒素蛋白及其制备工艺和医用用途，是一种预防和治疗隐孢子虫病的药物，利用单克隆抗体技术和基因重组技术，从分泌抗微小隐孢子虫子孢子表膜抗体的杂交瘤细胞株中克隆其可变区基因，再连接成单链抗体基因，然后将其插入原核表达载体的相应位点上，构建了重组原核表达质粒，再分别采用酶切和PCR方法将去除受体结合区的细菌外毒素插入重组原核质粒中单链抗体基因的下游位点，构建重组表达质粒。将上述构建的重组表达质粒分别转化受体菌，经IPTG诱导，SDS-PAGE分析表达产物，后对表达产物包涵体进行纯化和复性，并对其进行体外抗虫活性测定和模型动物隐孢子虫病治疗。 |
| 国际公布 |
