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您现在的位置: 医学全在线 >> 药学理论 >> 中药及天然药物质量控制研究方法 >> 正文:蚯蚓纤溶酶活性影响因子的研究中药质量控制方法/操作步骤
    

蚯蚓纤溶酶活性影响因子研究

引用本文:刘向辉;戈峰,.蚯蚓纤溶酶活性影响因子的研究[J].中国中药杂志,2002,27(06):423.

中文摘要:目的 :对提取过程中影响蚯蚓纤溶酶活性的 5种因子进行了分析 ,以提高纤溶酶的提取活性。方法 :分别采用 0.9%NaCl抽提、10%蔗糖抽提、75%精抽提出粗酶。酶活测定方法采用尿激酶琼脂糖 纤维蛋白平板法。硒含量的测定方法采用 3,3′-二基联苯胺比色法 ;砷含量的测定方法采用二乙氨基二硫代甲酸银比色法。结果 :饲养在牛粪饵料中的蚯蚓纤溶酶活性比饲养于生活垃圾中的蚯蚓纤溶酶活性更高 ,蚯蚓体内砷的含量与纤溶酶活性呈正相关 ,而蚯蚓体内硒的含量对纤溶酶活性影响不大。在采用 3种不同的蚯蚓纤溶酶的抽提方法中 ,75 %酒精抽提效率最高 ,0.9%氯化钠次之 ,10%蔗糖最低 ;在蚯蚓纤溶酶纯化过程中 ,透析比超滤对蚯蚓纤溶酶的活性影响更小一些。结论 :通过将蚯蚓饲养在适宜的饵料中 ,使用合适的提取手段和提纯方法 ,可以提高蚯蚓纤溶酶的提取活性。
中文关键词:蚯蚓纤溶酶活性  饵料  重金属  抽提方法

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Factors Influencing the Activity of Fibrinolytic Enzymes From Earthworm, Eisenia foetida
LIU Xiang hui;GE Feng
 
 
Abstract:Objective:To study the factors influencing the activity of fibrinolytic enzymes from earthworm and to obtain the better way to extract fibrinolytic enzymes as well as keep its optimum activity. Method:75% alcohol, 0.9%NaCl and 10% saccharose was used to extract the crude fibrinolytic enzymes from earthworm, the method of urokinase gelose fibrin plate was used to measure the activity of fibrinolytic enzymes from earthworm. and the method of 3,3′-diaminobezidine tetrahydrochloride colorimetry to was used measure the content of selenium. The method use ts of measuring the content of arsenic was silver diethyldithiocarbamate colorimetry. Result:The fibrinolytin of earthworms reared with cattle soils had higher activity than that reared with garbage. The arsenic in the earthworm′s body could improve the activity of earthworm′s fibrinolytin. However, the selenium had litter influence on it. Among the three methods of extraction, the 75% alcohol one was the most efficient , the 0.9% NaCl was next, and the 10% saccharose was the lowest. The influence of dialysis on the activity of fibrinolytin was less than that of ultrafiltration, when the earthworm′s fibrinolytin enzyme was further sublimated. Conclusion:The activity of the earthworm′s fibrinolytin will be increased earthworm is reared with the fitting baits and when appropriate methods, of extraction and purification are used.
keywords:activity of the earthworm′s fibrinolytin bait  heavy metal  method of extraction 
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参考文献(共7条):
[1]金鸣.蚯蚓溶栓作用进展.中草药,1994,24(1):45.
[2]路英华,金汝成,吴应文,等. 钜齿远蚓纤溶酶的分离纯化及其若干性质.生物化学杂志,1998,4(2):166.
[3]徐建民,张国桢,陈松鹤,等. 赤子爱胜蚓纤维蛋白溶解酶的亲和层析纯化和生化特性.上海医科大学学报,1991,18(4):252.
[4]Nakajima N, Mihara H, Sumi H. Characterization of Potent Fibrinolytic Enzymes in Earthworm Lumbricus Rubellus. Biochem, 1993,57(10):1726.
[5]李群. 蚓激酶及其制剂活性的研究. 中国药事, 1998,15(5):288.
[6]江苏省连云港水产学校. 水产品检验分析. 北京:中国农业出版社,1995.
[7]刘向辉,戈峰,徐张红,等. 亚硒酸钠对蚯蚓的毒性及其蚓体富硒作用的研究.应用与环境生物学报,2001,7(5):457.
 
引证文献(本文共被引2次):
[1] 武金霞,赵晓瑜.蚯蚓纤溶酶的研究进展及趋势[J].中国生化药物杂志,2004,25(5).
[2] 解佰纯,李景鹏.蚯蚓纤溶酶的研究进展[J].东北农业大学学报,2006,37(6):834-837.

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